phosphor ser396 s404 mouse (Cell Signaling Technology Inc)
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
95
Structured Review
Cell Signaling Technology Inc
phosphor ser396 s404 mouse
Phosphor Ser396 S404 Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+ser396+s404+mouse/Phospho-Tau+(Ser396)+Mouse+mAb/pmc06075360-158-94-88
Average 95 stars, based on 191 article reviews
Phosphor Ser396 S404 Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphor+ser396+s404+mouse/Phospho-Tau+(Ser396)+Mouse+mAb/pmc06075360-158-94-88
Average 95 stars, based on 191 article reviews
phosphor ser396 s404 mouse - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology"
Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology
Journal: Human Molecular Genetics
doi: 10.1093/hmg/ddx088
Figure Legend Snippet: Diet-induced high Hcy-dependent increase in tau phosphorylation is prevented by genetic absence of ALOX5. (A) Representative western blots of soluble total tau, phosphorylated tau at residues T231/S235 (AT180), at T181 (AT270), S396/S404 (PHF1) and S396 (PHF13) in brain cortex homogenates from wild type mice receiving regular diet (WT); wild type mice receiving folate and B vitamin deficient diet (WT + Diet); 5LOKO mice receiving regular diet (5LOKO); and 5LOKO mice receiving the Diet (5LOKO + Diet). (B) Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel (*P < 0.05). Values represent mean ± S.E.M. (n = 5 WT-control, n = 5 WT + Diet, n = 5 5LOKO-control, n = 5 5LOKO + Diet).
Techniques Used: Western Blot
Figure Legend Snippet: Antibodies used in the study
Techniques Used: Purification, Recombinant
Related Articles
Incubation:Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Imaging:Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Purification:Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Recombinant:Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing Article Title: Genetic absence of ALOX5 protects from homocysteine-induced memory impairment, tau phosphorylation and synaptic pathology Article Snippet: Briefly, samples were electrophoresed on 10% Bis–Tris gels or 3–8% Tris–acetate gel (Bio-Rad, Richmond, CA), transferred onto nitrocellulose membranes (Bio-Rad), and then incubated overnight with the appropriate primary antibodies as indicated in . .. After three washings with T-TBS (pH7.4), membranes were incubated with IRDye 800CW-labeled secondary antibodies (LI-COR Bioscience, Lincoln, NE) at RT for 1 h. Signals were developed with Odyssey Infrared Imaging Systems (LI-COR Bioscience). β-Actin was always used as internal loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Immunogen Host Dilution Source Catalog number Tau-1 Bovine microtubule associated protein Mouse 1:200 Cell signaling 4019 AT-180 Peptide containing phospho-T231/S235 Mouse 1:200 Thermo P10636 AT-270 Peptide containing phospho-T181 Mouse 1:300 Thermo MN1050 PHF-13 Peptide containing phospho-Ser396 Mouse 1:300 Cell signaling 9632 PHF-1 Peptide containing |